Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods

Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods PDF

By Francisco J. Pelegri

Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods PDF. One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar. These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development.  In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.

Read more: Lippincott’s Illustrated Q&A Review of Anatomy and Embryology

Table of Contents

Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis

Embryological and Genetic Manipulation of Chick Development

Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

Experimental Manipulation of Ploidy in Zebrafish Embryos and Its Application in Genetic Screens

RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos

Ex Utero Culture and Imaging of Mouse Embryos

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

Reptile Embryology and Regeneration

Manipulation of Developmental Function in Turtles with Notes on Alligators

Methods for Isolating the Balbiani Body/Germplasm from Xenopus laevis Oocytes

Visualizing the Balbiani Body in Zebrafish Oocytes

Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

Generation of Xenopus Haploid, Triploid, and Hybrid Embryos

Germ Cell Transplantation in Avian Species

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