Salmonella, Methods and Protocols, 2nd Edition

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
Table of Contents

Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 Luminex® Multiplex Bead Suspension Arrays for the Detection
and Serotyping of Salmonella spp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Sherry A. Dunbar, Vivette Brown Ritchie, Michaela R. Hoffmeyer,
Gunjot S. Rana, and Hongwei Zhang
2 Quantitative Proteomic Identification of Host Factors Involved
in the Salmonella typhimurium Infection Cycle . . . . . . . . . . . . . . . . . . . . . . . . 29
Dora Kaloyanova, Mijke Vogels, Bas W.M. van Balkom,
and J. Bernd Helms
3 Determination of Antimicrobial Resistance in Salmonella spp. . . . . . . . . . . . . . 47
Belgode N. Harish and Godfred A. Menezes
4 Red-Mediated Recombineering of Salmonella enterica Genomes . . . . . . . . . . . 63
Frederik Czarniak and Michael Hensel
5 A Method to Introduce an Internal Tag Sequence
into a Salmonella Chromosomal Gene. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Weidong Zhao and Stéphane Méresse
6 Generation and Use of Site-Directed Chromosomal cyaA′ Translational
Fusions in Salmonella enterica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Francisco Ramos-Morales, Elena Cardenal-Muñoz,
Mar Cordero-Alba, and Fernando Baisón-Olmo
7 Detection of Antimicrobial (Poly)Peptides with Acid Urea
Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot . . . . . . 105
Edith Porter, Erika V. Valore, Rabin Anouseyan, and Nita H. Salzman
8 Detecting Non-typhoid Salmonella in Humans
by Enzyme- Linked Immunosorbent Assays (ELISAs):
Practical and Epidemiological Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Katrin G. Kuhn, Hanne-Dorthe Emborg, Karen A Krogfelt,
and Kåre Mølbak
9 Study of the Stn Protein in Salmonella; A Regulator of Membrane
Composition and Integrity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Masayuki Nakano, Eiki Yamasaki, Joel Moss, Toshiya Hirayama,
and Hisao Kurazono
10 Development of a Bacterial Nanoparticle Vaccine . . . . . . . . . . . . . . . . . . . . . . 139
Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho,
and Juan M. Irache

11 Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium
Membranes Designed for Combination Bacteriotherapy Against Tumors. . . . . 151
Robert Kazmierczak, Elizabeth Choe, Jared Sinclair,
and Abraham Eisenstark
12 Applications of Microscopy in Salmonella Research . . . . . . . . . . . . . . . . . . . . . 165
Layla M. Malt, Charlotte A. Perrett, Suzanne Humphrey,
and Mark A. Jepson
13 Live Cell Imaging of Intracellular Salmonella enterica . . . . . . . . . . . . . . . . . . . 199
Alexander Kehl and Michael Hensel
14 In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization . . . 227
Geoffrey Gonzalez-Escobedo and John S. Gunn
15 Salmonella Phages and Prophages: Genomics, Taxonomy,
and Applied Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237
Andrea I. Moreno Switt, Alexander Sulakvelidze, Martin Wiedmann,
Andrew M. Kropinski, David S. Wishart, Cornelis Poppe,
and Yongjie Liang
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289

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