Salmonella, Methods and Protocols, 2nd Edition

Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 Luminex® Multiplex Bead Suspension Arrays for the Detection
and Serotyping of Salmonella spp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Sherry A. Dunbar, Vivette Brown Ritchie, Michaela R. Hoffmeyer,
Gunjot S. Rana, and Hongwei Zhang
2 Quantitative Proteomic Identification of Host Factors Involved
in the Salmonella typhimurium Infection Cycle . . . . . . . . . . . . . . . . . . . . . . . . 29
Dora Kaloyanova, Mijke Vogels, Bas W.M. van Balkom,
and J. Bernd Helms
3 Determination of Antimicrobial Resistance in Salmonella spp. . . . . . . . . . . . . . 47
Belgode N. Harish and Godfred A. Menezes
4 Red-Mediated Recombineering of Salmonella enterica Genomes . . . . . . . . . . . 63
Frederik Czarniak and Michael Hensel
5 A Method to Introduce an Internal Tag Sequence
into a Salmonella Chromosomal Gene. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Weidong Zhao and Stéphane Méresse
6 Generation and Use of Site-Directed Chromosomal cyaA′ Translational
Fusions in Salmonella enterica . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Francisco Ramos-Morales, Elena Cardenal-Muñoz,
Mar Cordero-Alba, and Fernando Baisón-Olmo
7 Detection of Antimicrobial (Poly)Peptides with Acid Urea
Polyacrylamide Gel Electrophoresis Followed by Western Immunoblot . . . . . . 105
Edith Porter, Erika V. Valore, Rabin Anouseyan, and Nita H. Salzman
8 Detecting Non-typhoid Salmonella in Humans
by Enzyme- Linked Immunosorbent Assays (ELISAs):
Practical and Epidemiological Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Katrin G. Kuhn, Hanne-Dorthe Emborg, Karen A Krogfelt,
and Kåre Mølbak
9 Study of the Stn Protein in Salmonella; A Regulator of Membrane
Composition and Integrity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Masayuki Nakano, Eiki Yamasaki, Joel Moss, Toshiya Hirayama,
and Hisao Kurazono
10 Development of a Bacterial Nanoparticle Vaccine . . . . . . . . . . . . . . . . . . . . . . 139
Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho,
and Juan M. Irache

11 Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium
Membranes Designed for Combination Bacteriotherapy Against Tumors. . . . . 151
Robert Kazmierczak, Elizabeth Choe, Jared Sinclair,
and Abraham Eisenstark
12 Applications of Microscopy in Salmonella Research . . . . . . . . . . . . . . . . . . . . . 165
Layla M. Malt, Charlotte A. Perrett, Suzanne Humphrey,
and Mark A. Jepson
13 Live Cell Imaging of Intracellular Salmonella enterica . . . . . . . . . . . . . . . . . . . 199
Alexander Kehl and Michael Hensel
14 In Vitro Modeling of Gallbladder-Associated Salmonella spp. Colonization . . . 227
Geoffrey Gonzalez-Escobedo and John S. Gunn
15 Salmonella Phages and Prophages: Genomics, Taxonomy,
and Applied Aspects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 237
Andrea I. Moreno Switt, Alexander Sulakvelidze, Martin Wiedmann,
Andrew M. Kropinski, David S. Wishart, Cornelis Poppe,
and Yongjie Liang
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289