Transgenic Mouse, Methods and Protocols, 2nd Edition
By Marten H. Hofker and Jan Deursen
Transgenic Mouse, Methods and Protocols, 2nd Edition PDF. The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models
Provides descriptions of how to generate conventional knockout and transgenic mice
Includes detailed protocols for vector preparation of different kinds of mouse models
Table of Contents
Introduction: Strategies for Developing Genetically Modified Mice
Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection
Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos
Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization
Autopsy and Histological Analysis of the Transgenic Mouse
Transgene Design
Inducible Transgenic Mouse Models
Lentiviral Transgenesis
Transgenesis in Mouse Embryonic Stem Cells
Engineering BAC Reporter Gene Constructs for Mouse Transgenesis
Targeting Vector Construction Through Recombineering
Generating Conditional Knockout Mice
Hypomorphic Mice
MICER Targeting Vectors for Manipulating the Mouse Genome
Knock-In Approaches
Generation of a Series of Knock-In Alleles Using RMCE in ES Cells
Selection of Targeted Mutants from a Library of Randomly Mutagenized ES Cells
Generation of Genetically Modified Rodents Using Random ENU Mutagenesis
Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells
