Transgenic Mouse, Methods and Protocols, 2nd Edition

By Marten H. Hofker and Jan Deursen

Transgenic Mouse, Methods and Protocols, 2nd Edition PDF. The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.

  • Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models
  • Provides descriptions of how to generate conventional knockout and transgenic mice
  • Includes detailed protocols for vector preparation of different kinds of mouse models
Table of Contents

Introduction: Strategies for Developing Genetically Modified Mice

Genetic Modification of the Mouse: General Technology – Pronuclear and Blastocyst Injection

Generation of Chimeras by Aggregation of Embryonic Stem Cells with Diploid or Tetraploid Mouse Embryos

Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

Autopsy and Histological Analysis of the Transgenic Mouse

Transgene Design

Inducible Transgenic Mouse Models

Lentiviral Transgenesis

Transgenesis in Mouse Embryonic Stem Cells

Engineering BAC Reporter Gene Constructs for Mouse Transgenesis

Targeting Vector Construction Through Recombineering

Generating Conditional Knockout Mice

Hypomorphic Mice

MICER Targeting Vectors for Manipulating the Mouse Genome

Knock-In Approaches

Generation of a Series of Knock-In Alleles Using RMCE in ES Cells

Selection of Targeted Mutants from a Library of Randomly Mutagenized ES Cells

Generation of Genetically Modified Rodents Using Random ENU Mutagenesis

Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

File Size7.5 MB
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