Transgenic Mouse, Methods and Protocols, 2nd Edition
The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man and because gene function studies in mice are in the context of a whole organism, making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenic Mouse Methods and Protocols, Second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockout mice.
- Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models
- Provides descriptions of how to generate conventional knockout and transgenic mice
- Includes detailed protocols for vector preparation of different kinds of mouse models