Mouse Cell Culture Methods and Protocols

Mouse Cell Culture Methods and Protocols PDF By Andrew Ward and David Tosh

By Andrew Ward and David Tosh

Mouse Cell Culture Methods and Protocols PDF describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

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Table of Contents

The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies

Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures

Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference

Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation

Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine

Isolation and Culture of Embryonic Pancreas and Liver

Isolation and Culture of Mouse Satellite Cells

Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures

Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes

Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells

Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

Isolation and Culture of Adult Mouse Hepatocytes

Isolation and Culture of Mouse Intestinal Cells

Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse

The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry

Cell Culture of Primary Cerebellar Granule Cells

Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain

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