Laboratory Fish in Biomedical Research, Biology, Husbandry and Research Applications for Zebrafish
By Samuel Cartner, Judith Eisen, Susan Farmer, Karen Guillemin, Michael Kent and George Sanders
Laboratory Fish in Biomedical Research, Biology, Husbandry and Research Applications for Zebrafish PDF. Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.
Provides husbandry and management protocols, devices and water systems
Shows strength and weakness of breeding
Explores potential scientific applications and experimental protocols. with regards to the most used freshwater fish used for scientific purposes
Table of Contents
Title page
Table of Contents
Copyright
Contributors
Fish as model systems
Section 1. Zebrafish (Danio rerio)
Chapter 1. Biology and research applications
The biology of the zebrafish
The history of the zebrafish as laboratory model
The zebrafish as a model for development and pathology
Chapter 2. Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design
Introduction to the model
Aquatic concepts
Establishing biofiltration
Husbandry
Chapter 3. Breeding and larviculture of zebrafish (Danio rerio)
Introduction
Should we mimic nature, or should we ignore it in laboratory breeding and husbandry of zebrafish: theoretical considerations from the perspective of experimental biology
Zebrafish in nature
Preparing zebrafish for breeding
The breeding tank
Raising zebrafish juveniles is not as easy as often stated
Hatching the eggs and caring for the larvae
Caring for young juveniles
Standardization of breeding and larviculture practices: the question of replicability and reproducibility
Chapter 4. Health monitoring, disease, and clinical pathology
Introduction
Controlling and monitoring population health
Pathogens and diseases to monitor
Fish and environmental samples
Diagnostic assays
Determining the number of samples
Introduction of zebrafish colonies and biosecurity
Conclusion
Chapter 5. The welfare of zebrafish
Introduction
Measuring welfare in zebrafish
Husbandry
Refinements in regulated procedures
Conclusion: the state of zebrafish welfare today
Chapter 6. Analgesia, anesthesia, and euthanasia in zebrafish
Analgesia
Anesthesia
Euthanasia
Conclusions
Chapter 7. Transgenesis, mutagenesis, knockdown, and genetic colony management
Introduction
Ethical issues in genetic manipulation of laboratory fish
Generation and management of morphants and CRISPants
Conclusions
Chapter 8. Sperm cryopreservation, in vitro fertilization, and embryo freezing
Introduction
Materials and methods
Conclusion
Section 2. Other small freshwater fish
Chapter 9. Medaka as a model teleost: characteristics and approaches of genetic modification
Medaka
Basic approaches of generation of transgenic/knockout medaka
Introduction of new transgenic methods
Chapter 10. Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation
Medaka as a powerful model animal for research fields of reproduction
Mechanisms of reproductive systems clarified by histological and physiological analyses
Histological studies using medaka
The present scheme of HPG axis regulation clarified by multidisciplinary analyses in vertebrates
Endocrine studies using medaka
Neurophysiological studies using medaka: patch clamp recording and calcium imaging
Medaka provides clues to the understanding of general mechanisms of reproduction in teleosts and even in vertebrates
Chapter 11. The African turquoise killifish (Nothobranchius furzeri): biology and research applications
Introduction
Large-scale husbandry (including water systems and water parameters)
Breeding strategies for genome engineering, including egg collection and incubation
Efficient genome engineering approaches
Fish dissection
The African turquoise killifish: available toolbox
Summary and future perspectives
Materials
Chapter 12. Challenges in keeping annual killifish
