Cellular and Molecular Approaches in Fish Biology PDF is a highly interdisciplinary resource to bring industry professionals, students and researchers up-to-date with the latest developments and information on fish biology research combining a historical overview of the different research areas in fish biology and detailed descriptions of cellular and molecular approaches with explanations and recommendations for research. The book presents a global perspective of each research area with detailed analytical methodologies on the cellular and molecular mechanisms within fish biology for experimentation. The book provides different points of view on how researchers have addressed timely issues, while describing and dissecting some of the new experimental/analytical approaches used to answer the key questions at cellular and molecular levels, making this a valuable resource to those in industry and academia as well as those entering the field.
Provides detailed descriptions of each research approach, highlighting the tricks of the trade for its effective and successful application
Includes the latest developments in fish reproduction, fish development and nutrition, fish welfare, fish immunology, ecology and biomedics
Presents hot topics of research such as genetics, transcriptomics and epigenetics
Table of Contents
Cover
Title page
Table of Contents
Copyright
Contributors
Editor’s biographies
Preface
Chapter 1: Technologies and strategies for ex situ conservation of aquatic organisms: The role of cryopreservation in long-term management
Abstract
Acknowledgments
1: Factors and steps to consider in setting up a cryopreservation protocol
2: Applications of cryopreservation techniques in aquatic animals
3: Cryopreservation of different biological materials to create Genome Resource Banks
4: Examples of sperm cryopreservation in specific species
5: Conclusions and future perspectives
References
Further reading
Chapter 2: Molecular approaches on DNA damage evaluation after primordial germ cell cryopreservation in zebrafish
Abstract
Acknowledgments
1: Introduction
2: Conventional DNA evaluation methods
3: DNA lesion detection by qPCR: A novel approach
4: Our contribution to DNA damage evaluation in cryopreserved germ cells
5: Specific protocol for DNA damage evaluation in zebrafish PGCs after cryopreservation
6: Summary and future perspectives
References
Chapter 3: The role of epigenetics in fish biology and reproduction: An insight into the methods applied to aquaculture
Abstract
1: Introduction: Concept and importance of epigenetics in terms of evolution
2: Epigenetic mechanisms
3: The role of epigenetics in farmed fish species
4: Epigenetic mechanisms in model species: A case study in zebrafish
5: Techniques applied for the evaluation of DNA methylation
6: Techniques applied for the evaluation of histone modifications
7: Application of the epigenetic methods to our research
References
Further reading
Chapter 4: Noncoding RNAs in fish physiology and development: miRNAs as a cornerstone in gene networks
Abstract
Acknowledgments
1: Noncoding RNAs
2: Biogenesis and mechanisms of action of miRNAs
3: Methodological approaches applied in miRNA research
4: miRNAs playing key roles in fish development and physiology
5: Recent progress and future perspectives
References
Chapter 5: Nutritional epigenetics
Abstract
1: Introduction
2: Epigenetic regulators
3: Nutrients and metabolites influencing the epigenetic regulators
4: Transfer of cell memory utilizing the epigenetic regulators
5: Where do we go with nutritional epigenetics?
References
Chapter 6: Methods for mapping genomic variants associated with production traits in aquaculture species
Abstract
1: Introduction
2: Finding causative variants through QTL mapping approaches
3: Detailed description of state-of-the-art methodology: Step by step on how to perform GWAS
4: Pipeline
5: The next generation of association mapping methods: Multimarker models
6: Implementation of QTLs and causative variants into breeding programs
7: Funneling candidate variants toward causality
8: Conclusions
References
Chapter 7: Double-labeling immunofluorescence: A simple and effective tool to study pituitary regulation in teleost fish
Abstract
Acknowledgments
1: The importance of gaining knowledge about the neuroendocrine system
2: Neuroendocrine system
3: Double-labeling immunofluorescence
4: Going deeper on the pituitary hormone regulation
5: How to approach this kind of neuroendocrine study in fish?
6: Summary
References
Chapter 8: Mucosal immunology in fish
Abstract
Acknowledgments
1: A brief introduction to the immune system in teleost fish
2: Historical perspective and relevance of immunological studies in gilthead seabream
3: Fish mucosal immunology
4: How to alter or strengthen the fish mucosal immune system
5: Methodologies to collect and preserve fish mucus
6: Main questions still to be addressed
References
Chapter 9: Characterization of the neuroendocrine stress status as part of the multiparametric assessment of welfare in fish
Abstract
1: Animal welfare
2: The neuroendocrine stress response and its relation to welfare
3: A proposed method to determine the stress status of fishes as part of the assessment of their welfare
4: Conclusions and future perspectives
References
Chapter 10: Evolution of ray-finned fish genomes: Status and directions with a primer on microRNA characterization
Abstract
1: Introduction
2: Resources to study fish genome evolution
3: Overview of fish genome evolution
4: Evolutionary consequences of rediploidization—No “one size fits all”
5: Transposable elements as agents of fish genome evolution
6: Fish phylogenomics
7: Evolution of “functional” regions in fish genomes
8: A primer on miRNA evolutionary characterization
9: Concluding remarks
References
Chapter 11: Zebrafish xenotransplantation: A versatile platform for cancer and stem cell translational research
Abstract
1: Introduction
2: Larval zebrafish XT models of cancer
3: Using the zebrafish for translational stem cell research
4: Adult zebrafish XT models of cancer
5: Conclusion
References
Chapter 12: Status, challenges, and perspectives of fish cell culture—Focus on cell lines capable of in vitro mineralization
Abstract
Acknowledgments
1: A growing interest for cell lines of fish origin
2: Current status of fish cell culture
3: Applications for fish cell lines
4: Fish cell lines capable of in vitro mineralization
