Mouse Genetics Methods and Protocols

Mouse Genetics Methods and Protocols PDF

By Shree Ram Singh and Vincenzo Coppola

Mouse Genetics: Methods and Protocols PDF provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Read more: Laboratory Animal Law: Legal Control of the Use of Animals in Research, 2nd Edition

Table of Contents

Mouse Genetics Techniques

Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination

Generation of Conditional Knockout Mice

Generating Mouse Lines for Lineage Tracing and Knockout Studies

Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)

Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection

Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases

Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice

Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

Generation of Genetically Engineered Mice by the piggyBac Transposon System

Generation and Applications of MADM-Based Mouse Genetic Mosaic System

Generation of Mouse Lines Conditionally Over-expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System

In Situ Hybridization (Both Radioactive and Nonradioactive) and Spatiotemporal Gene Expression Analysis

Mouse Genetics in Stem Cells

Isolation and Handling of Mouse Embryonic Fibroblasts

Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells

Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System

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