Diagnostic Tools In Veterinary Medicine

Diagnostic Tools In Veterinary Medicine

By Patricia Marques

The Key Components of Veterinary Diagnostic System are Vet Laboratory, Vet Devices and Veterinary Diagnostic Test Kits. Traditionally, laboratory diagnostics for veterinary pathogens have relied on methods of detecting the pathogen by culture or antibodies, using varied techniques, such as neutralization, Enzyme-linked Immunosorbent Assay (ELISA), agar gel immunodiffusion, and complement fixation. Over the years, veterinarians have incorporated new molecular diagnostic techniques, such as Polymerase Chain Reaction (PCR) and Western blot, as well as improved older techniques by using recombinant antigens, monoclonal antibodies, and synthetic peptides. Despite the routine use of conventional diagnostic assays, new molecular techniques have enabled veterinarians to use new tools for rapid and specific diagnosis of animal disease in real-time. The globalization of trade in animals and animal products is a major driver for the improvement of the analytical and diagnostic quality of tests.

Table of Contents
  1. Epidemiology and Surveillance
    • Chapter 1: Identifying an Outbreak of a Novel Swine Disease using Test Requests for Porcine Reproductive and Respiratory Syndrome as a Syndromic Surveillance Tool
    • Chapter 2: Syndromic Surveillance Using Veterinary Laboratory Data: Algorithm Combination and Customization of Alerts
    • Chapter 3: A Focused Ethnographic Study of Alberta Cattle Veterinarians’ Decision Making about Diagnostic Laboratory Submissions and Perceptions of Surveillance Programs
    • Chapter 4: Exploratory Analysis of Methods for Automated Classification of Laboratory Test Orders into Syndromic Groups in Veterinary Medicine
  2. Serology
    • Chapter 5: Getting More Out of Less – A Quantitative Serological Screening Tool for Simultaneous Detection of Multiple Influenza A Hemagglutinin-Types in Chickens
    • Chapter 6: Serological Diagnostic Potential of Recombinant Outer Membrane Proteins (rOMPs) from Brucella Melitensis in Mouse Model using Indirect Enzyme-linked Immunosorbent Assay
  3. Immunoassays
    • Chapter 7: Comparison of a Flow Assay for Brucellosis Antibodies with the Reference CELISA Test in West African Bos indicus
  4. Tissue Culture
    • Chapter 8: Proof of Concept of Faecal Egg Nematode Counting as a Practical Means of Veterinary Engagement with Planned Livestock Health Management in a Lower Income Country
    • Chapter 9: Diagnosis of Bacterial Urinary Tract Infection: Utility of Urine Myeloperoxidase Concentration to Predict Urine Culture Results in Dogs
  5. Diagnostic using PCR and Sequencing Techniques
    • Chapter 10: Transcriptomic Markers Meet the Real World: Finding Diagnostic Signatures of Corticosteroid Treatment in Commercial Beef Samples
    • Chapter 11: A Molecular Diagnostic Tool to Replace Larval Culture in Conventional Faecal Egg Count Reduction Testing in Sheep
    • Chapter 12: BeeDoctor, a Versatile MLPA-Based Diagnostic Tool for Screening Bee Viruses
  6. Diagnosis by Imaging
    • Chapter 13: Confocal Laser Scanning Microscopy, a New in Vivo Diagnostic Tool for Schistosomiasis
    • Chapter 14: Rhinoceros Feet Step Out of a Rule-of-Thumb: A Wildlife Imaging Pioneering Approach of Synchronized Computed Tomography-Digital Radiography
    • Chapter 15: International Veterinary Epilepsy Task Force Consensus Reports on Epilepsy Definition, Classification and Terminology, Affected Dog Breeds, Diagnosis, Treatment, Outcome Measures of Therapeutic Trials, Neuroimaging and Neuropathology in Companion Animals
  7. Mass Spectrometry
    • Chapter 16: Quantitative and Selective Analysis of Feline Growth Related Proteins Using Parallel Reaction Monitoring High Resolution Mass Spectrometry
    • Chapter 17: MALDI-TOF Mass Spectrometry as a Diagnostic Tool in Human and Veterinary Helminthology: A Systematic Review
    • Chapter 18: Peptide Barcodes in Dogs affected by Mitral Valve Disease with and without Pulmonary Hypertension using MALDI-TOF MS and LC-MS/MS
  8. Specific Instruments for Veterinary Diagnosis
    • Chapter 19: Diagnostic Accuracy of a Short-duration 3 Tesla Magnetic Resonance Protocol for Diagnosing Stifle Joint Lesions in Dogs with Non-traumatic Cranial Cruciate Ligament Rupture
    • Chapter 20: Evaluation of a New Portable 1-lead Digital Cardiac Monitor (eKuore) compared with Standard Base-apex Electrocardiography in Healthy Horses
  9. Machine Learning and AI
    • Chapter 21: Validation of Computerized Diagnostic Information in a Clinical Database from a National Equine Clinic Network
    • Chapter 22: Proof of Concept of a Workflow Methodology for the Creation of basic Canine head Anatomy Veterinary Education Tool Using Augmented Reality
  10. Other Methods of Diagnosis
    • Chapter 23: Novel Physico-chemical Diagnostic Tools for High Throughput Identification of Bovine Mastitis Associated Gram-Positive, Catalase-negative Cocci
    • Chapter 24: The Usefulness of Uterine Lavage and Acute Phase Protein Levels as a Diagnostic Tool for Subclinical Endometritis in Icelandic Mares
    • Chapter 25: Diagnostic Tools of Caprine and ovine Anaplasmosis: A Direct Comparative Study

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