Proteins: Biochemistry and Biotechnology 2nd Edition

1126
By Gary Walsh

Proteins: Biochemistry and Biotechnology 2nd Edition

Proteins: Biochemistry and Biotechnology, 2nd Edition PDF is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine.

Fully updated throughout with  new or fundamentally revised sections  on proteomics as, bioinformatics, protein glycosylation and  engineering,  well as sections detailing advances in upstream processing and  newer protein  applications such as enzyme-based biofuel production  this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies.

Proteins: Biochemistry and Biotechnology 2nd Edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins.

Proteins: Biochemistry and Biotechnology 2nd Edition Features:

  • Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology
  • Includes numerous case studies
  • Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology
  • Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production
Table of Contents

Proteins: Biochemistry and Biotechnology 2nd Edition Table of Contents

Preface xi

About the Companion Website xiii

Chapter 1 Proteins and proteomics 1

1.1 Proteins, an introduction 1

1.2 Genes, genomics and proteomics 2

1.3 Bioinformatics 12

1.4 Proteomics: goals and applications 14

Further reading 22

Chapter 2 Protein structure and engineering 25

2.1 Primary structure 25

2.2 Higher-level structure 36

2.3 Protein classification on the basis of structure 41

2.4 Protein structural stability 45

2.5 Higher-order structure prediction 47

2.6 Protein folding 48

2.7 Intrinsically disordered proteins 50

2.8 Protein engineering 51

2.9 Protein post-translational modification 54

Further reading 62

Chapter 3 Protein sources 65

3.1 Recombinant versus non-recombinant production 65

3.2 Approaches to recombinant protein production 67

3.3 Heterologous protein production in E. coli 72

3.4 Heterologous production in bacteria other than E. coli 77

3.5 Heterologous protein production in yeast 77

3.6 Heterologous protein production in fungi 78

3.7 Proteins from plants 80

3.8 Animal tissue as a protein source 84

3.9 Heterologous protein production in transgenic animals 85

3.10 Heterologous protein production using animal cell culture 86

3.11 Insect cell culture systems 87

Chapter 4 Protein purification and characterization 91

4.1 Protein detection and quantification 93

4.2 Initial recovery of protein 95

4.3 Removal of whole cells and cell debris 98

4.4 Concentration 103

4.5 Chromatographic purification 107

4.6 Protein inactivation and stabilization 128

4.7 Protein characterization 137

Further reading 139

Chapter 5 Large-scale protein production 141

5.1 Upstream processing 141

5.2 Downstream processing 154

5.3 Therapeutic protein production: some special issues 163

5.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166

Further reading 175

Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 177

6.1 Blood products 177

6.2 Anticoagulants 184

6.3 Thrombolytic agents 186

6.4 Additional blood-related products 189

6.5 Vaccine technology 190

6.6 Therapeutic enzymes 194

Further reading 202

Chapter 7 Therapeutic antibodies 205

7.1 Antibodies 205

7.2 IgG structure and activity 205

7.3 Antibody therapeutics: polyclonal antibody preparations 209

7.4 Antibody therapeutics: monoclonal antibodies 211

7.5 Therapeutic applications of monoclonal antibodies 220

7.6 Antibody conjugates 223

7.7 Bispecific antibodies 224

7.8 Antibody fragments 225

7.9 Engineering the antibody glycocomponent 228

7.10 Fc fusion proteins 229

Further reading 230

Chapter 8 Hormones and growth factors used therapeutically 233

8.1 Insulin 233

8.2 Glucagon 240

8.3 Gonadotrophins 240

8.4 Growth hormone 243

8.5 Erythropoietin 246

8.6 Other hormones 247

8.7 Growth factors 249

Further reading 253

Chapter 9 Interferons, interleukins and tumour necrosis factors 257

9.1 Regulatory factors: cytokines versus hormones 257

9.2 Interferons 258

9.3 Interleukins 264

9.4 Tumour necrosis factors 271

Further reading 274

Chapter 10 Proteins used for analytical purposes 277

10.1 The IVD sector 279

10.2 The basis of analyte detection and quantification 280

10.3 Enzymes as diagnostic/analytical reagents 281

10.4 Biosensors 289

10.5 Antibodies as analytical reagents 295

Further reading 309

Chapter 11 Industrial enzymes: an introduction 311

11.1 Sales value and manufacturers 313

11.2 Sources and engineering 314

11.3 Environmental benefits 315

11.4 Enzyme detection and quantification 315

11.5 Immobilized enzymes 316

11.6 Extremophiles 319

11.7 Enzymes in organic solvents 324

11.8 Industrial enzymes: the future 325

Further reading 325

Chapter 12 Industrial enzymes: proteases and carbohydrases 327

12.1 Proteolytic enzymes 327

12.2 Carbohydrases 340

Further reading 367

Chapter 13 Additional industrial enzymes 371

13.1 Lipases 371

13.2 Penicillin acylase 375

13.3 Amino acylase and amino acid production 378

13.4 Cyclodextrins and cyclodextrin glycosyltransferase 380

13.5 Enzymes and animal nutrition 382

13.6 Enzymes in molecular biology 387

Further reading 390

Chapter 14 Non-catalytic industrial proteins 393

14.1 Functional properties of proteins 393

14.2 Milk and milk proteins 397

14.3 Animal-derived proteins 408

14.4 Plant-derived proteins 411

14.5 Sweet and taste-modifying proteins 412

Further reading 414

Index 417

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