Protein Phosphatase Protocols

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Protein Phosphatase Protocols

By Greg Moorhead

 

Table of Contents

Protein Phosphatase Protocols Contents

Chapter 1: Analysis of Protein Phosphatases: Toolbox For Unraveling Cell Signaling Networks

Chapter 2: A Brief Introduction to the Protein Phosphatase Families

Chapter 3: Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases: Specificity, Use, and Common Forms of Abuse

Chapter 4: Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin–Sepharose and Microcystin–Biotin–Sepharose

Chapter 5: Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated (ATM)

Chapter 6: An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity

Chapter 7: An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species

Chapter 8: Mutagenesis and Expression of the Scaffolding Aα and Aβ Subunits of PP2A: Assays for Measuring Defects in Binding of Cancer-Related Aα and Aβ Mutants to the Regulatory B and Catalytic C Subunits

Chapter 9: Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits

Chapter 10: Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies

Chapter 11: Purification of PP2Ac from Bovine Heart

Chapter 12: Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions

Chapter 13: Yeast Two-Hybrid Screens To Identify Drosophila PP1-Binding Proteins

Chapter 14: Identification of Cellular Protein Phosphatase-1 Regulators

Chapter 15: Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) with Regulatory Subunits Plus Phosphatase Inhibitor-2

Chapter 16: Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C

Chapter 17: Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin

Chapter 18: Proteins Interacting with Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry

Chapter 19: Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity

Chapter 20: The Use of RNA Interference to Analyze Protein Phosphatase Function In Mammalian Cells

Chapter 21: Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases

Chapter 22: Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases

Chapter 23: Targeting of PP2C in Budding Yeast

Chapter 24: Phosphatase Targets in TOR Signaling

Chapter 25: Functional Characterization of the Small CTD Phosphatases

Chapter 26: Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences: An Example Using the Protein Phosphatases of Arabidopsis thaliana

Chapter 27: Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases

Index

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