Methods in Reproductive Aquaculture, Marine and Freshwater Species


Methods in Reproductive Aquaculture, Marine and Freshwater Species

Methods In Reproductive Aquaculture, Marine And Freshwater Species

By Elsa Cabrita, Vanesa Robles and Paz Herraez

The large amount of information on fish reproduction available is not always readily accessible to all interested parties. Written to appeal to aquaculturalists, conservation managers, and scientific researchers, Methods in Reproductive Aquaculture provides an overview of available techniques and addresses ways to improve depleted stocks of endangered fish and other species.

The book focuses on gamete quality and management, exploring new objectives and areas of application and research, and new tools to deal with some aspects in reproduction of aquatic species. It begins with an examination of basic methods and techniques for gamete extraction, mainly sperm collection and egg stripping in different species, then describes techniques of spawning stimulation in males and females. The volume then highlights sperm and egg quality evaluation and considers sperm analysis from a practical broodstock management point of view and in the selection of appropriate breeders. The book goes on to discuss techniques used for artificial fertilization and the procedures for obtaining modified offspring. It introduces cryopreservation procedures for sperm, oocytes, and embryos, then discusses the materials and facilities required for gamete preparation and freezing-thawing procedures. The book concludes with an extensive section detailing sperm cryopreservation protocols for fifty-six marine and freshwater species.

Unique in its focus on both marine and freshwater aquaculture of fishes, the book provides information on endangered, highly profitable species for aquaculture or fisheries and species with high potential in laboratory research. Presenting the available information in an easily understandable way, this is not merely a techniques book, but also a complete guide to fish reproduction, gamete quality, and management.

Table of Contents


Reproduction and control of ovulation, spermiation and spawning in cultured fish, E. Mañanós, N. Duncan, and C. Mylonas

Methods for sperm collection, J-C. Gwo


Sperm quality assessment, E. Cabrita, V. Robles, and P. Herráez

Egg quality determination in teleost fish, F. Lahnsteiner, F. Soares, L. Ribeiro, and M.T. Dinis


Artificial fertilization in aquaculture species: from normal practice to chromosome manipulation, B. Urbányi , Á. Horváth, and Z. Bokor

SPERM AND EGG CRYOPRESERVATIONChilled storage of sperm and eggs, J. Bobe and C. Labbé

Basic principles of fish spermatozoa cryopreservation, J. Cloud and S. Patton

Cryopreservation of fish oocytes, T. Zhang and E. Lubzens

Embryo cryopreservation: what we know until now, V. Robles, E. Cabrita, J.P. Acker, and P. Herráez

Cryobiological material and handling procedures, F. Martínez-Pastor and S.L. Adams


Cryopreservation of sperm of loach (Misgurnus fossilis), J. Kopeika, E. Kopeika, T. Zhang, and D. Rawson

Zebrafish Sperm Cryopreservation with N, N-Dimethylacetamide, J.P. Morris IV, A. Hagen, and J.P. Kanki

Sperm cryopreservation of live-bearing fishes of the Genus Xiphophorus, Q. Dong, C. Huang, L. Hazlewood, R.B. Walter, and T.R. Tiersch

Use of post-thaw silver carp (Hypophtalmichthys molitrix) spermatozoa to increase hatchery productions, B. Alvarez, A. Arenal, R. Fuentes, R. Pimentel, Z. Abad, and E. Pimentel

Cryopreservation of common carp sperm, B. Urbányi and Á. Horváth

Sperm cryopreservation from sea perch, Lateolabrax japonicas, S-L. Chen, X.S. Ji, and Y-S. Tian

Semen cryopreservation of piracanjuba (Brycon orbignyanus), an endangered Brazilian species, A.T.M. Viveiros and A.N. Maria

Cryopreservation of endangered Formosan landlocked salmon (Oncorhynchus masou formosanus) semen, J-C. Gwo, H. Ohta, K. Okuzawa, L-Y. Liao, and Y-F. Lin

Protocols for the cryopreservation of Salmonidae semen, Lota lota (Gadidae) and Esox lucius (Esocidae), F. Lahnsteiner and N. Mansour

Rainbow trout (Oncorhynchus mykiss) sperm cryopreservation, P. Herráez, V. Robles, and E. Cabrita

Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss), V. Robles, E. Cabrita, and P. Herráez

Cryopreservation of testicular sperm from European catfish (Silurus glanis), G. Maisse, B. Ogier de Baulny, and C. Labbé

Semen Cryopreservation of the African catfish, Clarias gariepinus, A.T.M. Viveiros and J. Komen

Cryopreservation of sperm from species of the order Acipenseriformes, Á. Horváth, B. Urbányi, and S.D. Mims

Different protocols for the cryopreservation of European eel (Anguilla anguilla) sperm, J.F. Asturiano

Cryopreservation of Striped Bass Morone saxatilis Spermatozoa, L.C.Woods III, S. He, and K. Jenkins-Keeran

Cryopreservation of semen from striped trumpeter (Latris lineata), A.J. Ritar

Cryopreservation of Atlantic halibut sperm, Hippoglossus hippoglossus, I. Babiak

Sperm cryopreservation: an actual tool for Seabass (Dicentrarchus labrax) reproduction and breeding control in research and production, C. Fauvel and M. Suquet

Notes on Diplodus puntazzo sperm cryopreservation, F. Barbato, S. Canese, F. Moretti, A. Taddei, A. Fausto, L. Abelli, M. Mazzini, and K.J. Rana

Sperm Cryopreservation from the Marine Teleost, Sparus aurata, E. Cabrita, V. Robles, C. Sarasquete, and P. Herráez

Cryopreservation of seabream semen, J-C. Gwo

Cryopreservation of sperm from winter flounder, Pseudopleuronectes americanus, R.M. Rideout, I.A.E. Butts, and M.K. Litvak

Cryopreservation of sperm in turbot (Psetta maxima), M. Suquet, O. Chereguini, and C. Fauvel

Cryopreservation of grouper semen, J-C. Gwo and H. Ohta

Cryopreservation of sperm from Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus), R.M. Rideout and D.L. Berlinsky

Cryopreservation of small abalone (Haliotis diversicolor supertexa) semen, J-C. Gwo

Cryopreservation of Pacific Oyster Sperm, Q. Dong, C. Huang, B. Eudeline, and T. R. Tiersch

A simple method for freezing Pacific oyster (Crassostrea gigas) sperm in quantities suitable for commercial hatcheries, S.L. Adams, J.F. Smith, R.D. Roberts, A.R. Janke, H.F. Kaspar, H.R. Tervit, P.A. Pugh, S.C. Webb, and N.G. King

Cryopreservation of Eastern Oyster Sperm, C.G. Paniagua-Chavez and T.R. Tiersch

Cryopreservation of sea urchin (Evechinus chloroticus) spermS.L. Adams, P.A. Hessian, and P.V. Mladenov

Protocol for cryopreservation of Penaeus vannamei sperm cells, M. Salazar, M.Lezcano, and C.Granja

Techniques for cryopreservation of spermatophores of the giant freshwater prawn, Macrobrachium rosenbergii (de Man), P. Damrongphol and K. Akarasanon

Protocol development for the cryopreservation of spermatozoa and spermatophores of the mud crab, Scylla serrata, S. Bhavanishankar and T. Subramoniam


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