Experimental Surgical Models in the Laboratory Rat


Experimental Surgical Models in the Laboratory Rat

Experimental Surgical Models In The Laboratory Rat

By Alfredo Rigalli and Veronica Di Loreto

An All-Inclusive Guide to Surgical Techniques on Rats

The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.

Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.

CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction

With succinct descriptions, color photographs, and easy-to-follow steps, the attached CD-ROM is a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for today’s biomedical sciences investigator.

  • Includes an abundance of experimental models to study the different systems of the rat in normal or pathological situations
  • Contains a complete description of the operating theatre needed to perform the surgery
  • Discusses pathophysiologic situations in which each surgery is useful
  • Documents experimental results obtained with each surgery
  • Supplies a CD-ROM with more than 250 illustrations, most in color, that demonstrate each surgery technique in step-by-step fashion
Table of Contents

Section I: Introduction

Bioethics and Animal Care

Control of Stress and Distress

Management of Pain in Laboratory Animals

Anesthesia and Analgesia



Section II: General Procedures

Operating Theatre

Rat Identification

Antisepsis, Sterilization, and Asepsis, Diego Holotte


Substances Administration


Section III: Catheterism and Cannulation

Catheterism of the Femoral Artery and Vein

Cardiac Catheterization

Cannulation of the Thoracic Duct


Section IV: Gastrointestinal Tract

In Situ Isolation of the Stomach

In Situ Isolation of the Intestinal Loop

Intestinal Everted

In Situ Perfusion of the Small Intestine

Section V: Pancreas

Experimental Models for the Study of Diabetes

Islets of Langerhans Isolation

Incubation of Pancreatic Tissue Slices

In Situ Perfusion of The Pancreas

Experimental Pancreatitis

Section VI: Liver

Hepatic Circulation Impairment

Extrahepatic Cholestasis Model

Section VII: Ablation of Endocrine Glands


Ovariectomy, Verónica Di Loreto


Parathyroid Glands

Section VIII: Kidneys

Measurement Techniques of Renal Parameters

Kidney Isolation and Perfusion

Acute Renal Failure Models

Renal Blood Flow Measurement

Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex

Remnant Kidney Model

Section IX: Circulatory System

Experimental Myocardial Infarction

Experimental Arteriosclerosis

Isolated Heart: Langendorff Technique

Section X: Additional Techniques

Polyclonal Antibodies Preparation

Histological Procedures

Bones and Bone Tissue

Section XI: Miscellaneous


Solutions Preparation

Normal Values In Iim/Fm Rat Subline “M”


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