Cryptosporidium, Methods and Protocols

Cryptosporidium Methods and Protocols Book PDF

By Jan R. MeadMichael J. Arrowood

Cryptosporidium Methods and Protocols Book PDF encompass broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon.

Read more: Parasitic Zoonoses

Table of Contents

About Author 

Jan R. Mead: Atlanta Veterans Affairs Medical CenterDecaturUSA

Michael J. Arrowood: Division of Foodborne, Waterborne, and Environmental DiseasesNational Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and PreventionAtlantaUSA

Table of Contents

Front Matter

Cryptosporidium Diagnostic Assays: Microscopy

Cryptosporidium Diagnostic Assays: Molecular Detection

Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-
Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation

Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay

Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

Cryptosporidium Genotyping for Epidemiology Tracking

Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection

Preparation of Cryptosporidium DNA for Whole Genome Sequencing

Accessing Cryptosporidium Omic and Isolate Data via

A Novel Method to Silence Genes in Cryptosporidium

Use of miRNAs to Study Host Cell–Parasite Interactions

Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology

Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics

Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

High-Content Screening for Cryptosporidium Drug Discovery

High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR

In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds

In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers

Two- and Three-Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium

Correction to: Accessing Cryptosporidium Omic and Isolate Data via

Back Matter

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