By Rajib Deb, Ajay Kumar Yadav, Swaraj Rajkhowa and Yashpal Singh Malik
Protocols for the Diagnosis of Pig Viral Diseases PDF explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields.
Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.
Compiles various biotechnological tools and assays for the detection of both antigens and antibodies
Provides technically sound protocols for newer diagnostics tools
Contains problems encountered while using diagnostic tools as well as probable solutions with examples
Table of Contents
Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms
Collection of Samples, Their Preservation and Transportation
Methods for Quantification of Viruses
Protocols for Isolation of Genetic Materials from RNA Viruses
Multiplex PCR for Diagnosis of Porcine Diseases
Protocols for Isolation of Plasmid DNA
Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases
RNA-PAGE-Based Diagnosis of Viral Diseases
Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases
Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases
Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance
Antibody-Based Sensors for Pathogen Detection
Lateral Flow Assay for Diagnosis of Pig Viral Diseases
Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases
Protocols for Immunofluorescence Techniques
Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases
Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases
Cell Culture System for Porcine Virus Isolation and Propagation
An Overview of Mouse Monoclonal Antibody Production
Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective
