Protocols for the Diagnosis of Pig Viral Diseases

Protocols for the Diagnosis of Pig Viral Diseases PDF

By Rajib Deb, Ajay Kumar Yadav, Swaraj Rajkhowa and Yashpal Singh Malik

Protocols for the Diagnosis of Pig Viral Diseases PDF explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields.

Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

  • Compiles various biotechnological tools and assays for the detection of both antigens and antibodies
  • Provides technically sound protocols for newer diagnostics tools
  • Contains problems encountered while using diagnostic tools as well as probable solutions with examples
Table of Contents

Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms

Collection of Samples, Their Preservation and Transportation

Methods for Quantification of Viruses

Protocols for Isolation of Genetic Materials from RNA Viruses

Multiplex PCR for Diagnosis of Porcine Diseases

Protocols for Isolation of Plasmid DNA

Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases

RNA-PAGE-Based Diagnosis of Viral Diseases

Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases

Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases

Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance

Antibody-Based Sensors for Pathogen Detection

Lateral Flow Assay for Diagnosis of Pig Viral Diseases

Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases

Protocols for Immunofluorescence Techniques

Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases

Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases

Cell Culture System for Porcine Virus Isolation and Propagation

An Overview of Mouse Monoclonal Antibody Production

Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective

Ligase Detection Reaction-Fluorescent Microsphere Assay

ELISA as a Diagnostic Weapon

SDS-PAGE and Western Blotting: Basic Principles and Protocol

Immune Assays as Diagnostic for Pig Viral Diseases

Production of Virus-like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis

Good Laboratory Practices and Biosafety Containments in a Virology Laboratory

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