Animal Coronaviruses, 2nd Edition

By Leyi Wang

Animal Coronaviruses, 2nd Edition PDF provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Table of Contents

Preface
Contributors
PART1 REVIEWS
1 Animal Coronaviruses

2 Feline Coronavirus: Insights into the Pathogenesis and Diagnosis and Chengming Wang

3 Animal Models for Studying COVID-19 Pathology and Infection

4 Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples

5 Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells

6 Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs

7 Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs

8 Real-Time Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens

9 TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus

10 Real-Time RT-PCR for Detection of Feline Coronavirus

11 SARS-CoV-2 Real-Time RT-PCR Assay in Animals.

12 Real-Time PCR and Antigen-Capture ELISA for the Diagnosis

PART IV SEROLOGY
13 Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology

14 Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope
15 ELISA, Virus Neutralization, and Immunofluorescence Assays for
Detection of Antibodies Against Porcine Deltacoronavirus.

16 Luminescent Immunoprecipitation System (LIPS) Development for the bSpecific Detection of PDCoV and SARS-CoV-2 Antibodies

17 Construction of a Mouse-Feline Chimeric Neutralizing Antibode Against SARS-CoV-2

18 Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells

PARTV DISEASE-TARGETED ANIMAL SPECIES AS ANIMAL MODELS

19 Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices

20 Diagnostic Investigation and Confirmation of Emerging Turkey
Coronaviral Enteritis

PART VI NEXT-GENERATION SEQUENCING AND BIOINFORMATICS

21 Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus
and Porcine Deltacoronavirus

22 Whole Genome Sequencing of Avian Infectious Bronchitis Virus
by iSeq100 Platform and MinION

23 Phylogenetic and Recombination Analysis of Animal Coronaviruses

Index

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