Animal Coronaviruses, 2nd Edition PDF provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.
2 Feline Coronavirus: Insights into the Pathogenesis and Diagnosis and Chengming Wang
3 Animal Models for Studying COVID-19 Pathology and Infection
4 Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples
5 Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells
6 Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs
7 Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs
8 Real-Time Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens
9 TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus
10 Real-Time RT-PCR for Detection of Feline Coronavirus
11 SARS-CoV-2 Real-Time RT-PCR Assay in Animals.
12 Real-Time PCR and Antigen-Capture ELISA for the Diagnosis
PART IV SEROLOGY
13 Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology
14 Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope
15 ELISA, Virus Neutralization, and Immunofluorescence Assays for
Detection of Antibodies Against Porcine Deltacoronavirus.
16 Luminescent Immunoprecipitation System (LIPS) Development for the bSpecific Detection of PDCoV and SARS-CoV-2 Antibodies
17 Construction of a Mouse-Feline Chimeric Neutralizing Antibode Against SARS-CoV-2
18 Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells
PARTV DISEASE-TARGETED ANIMAL SPECIES AS ANIMAL MODELS
19 Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices
20 Diagnostic Investigation and Confirmation of Emerging Turkey
Coronaviral Enteritis
PART VI NEXT-GENERATION SEQUENCING AND BIOINFORMATICS
21 Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus
and Porcine Deltacoronavirus
22 Whole Genome Sequencing of Avian Infectious Bronchitis Virus
by iSeq100 Platform and MinION
23 Phylogenetic and Recombination Analysis of Animal Coronaviruses
