Laboratory Handbook on Bovine Mastitis, 3rd Edition

The National Mastitis Council (NMC) released its third edition of the Laboratory Handbook on Bovine Mastitis. A 148-page book for laboratory diagnosticians, veterinarians, educators, students, researchers, technical specialists and dairy producers, it discusses the basic theories of microbial culture, mastitis diagnosis and pathogens, laboratory procedures and molecular pathogen diagnosis methods. Previous editions were published in 1987 and 1999.

The new Chapter 4 addresses molecular techniques. These techniques are the most recent development in pathogen diagnosis and are becoming more accurate in identifying causative organisms by genus and species, as well as strain type. Mycoplasmas are now discussed in their own chapter – Chapter 8 – instead of with miscellaneous organisms. And, the new Chapter 12 covers on-farm culture systems.

Users of the previous edition will find the current book’s format similar to the second edition. Microbial culture remains the backbone of mastitis diagnosis. Consequently, microbial culture methods are discussed and have been updated when appropriate. The book addresses the advantages and disadvantages of various techniques in terms of accuracy, specificity and sensitivity of detection, cost and turnaround time.

The Laboratory Handbook on Bovine Mastitis includes information, photographs and illustrations, regarding sample collection and handling, diagnostic equipment and materials, diagnostic procedures, molecular diagnostics, mastitis pathogens, somatic cell count, bulk tank culture and on-farm culture. Also, the book contains appendices on general isolation media, mycoplasma medium and testing procedures, other media, testing procedures and stains.

Writing committee members for the third edition of Laboratory Handbook on Bovine Mastitis included Pamela Adkins, University of Missouri, Columbia, Mo.; Lawrence Fox, Washington State University, Pullman, Wash.; Sandra Godden, University of Minnesota, St. Paul, Minn.; Bhushan M. Jayarao, Pennsylvania State University, University Park, Pa.; Greg Keefe, Atlantic, Veterinary College, Charlottetown, P.E.I., Canada; David Kelton, University of Guelph, Guelph, Ont., Canada; Alfonso Lago, Dairy Experts, Tulare, Calif.; John Middleton, University of Missouri, Columbia, Mo.; William Owens, Louisiana State University, Homer, La.; Christina Petersson-Wolfe, Virginia Tech, Blacksburg, Va.; Gina M. Pighetti, University of Tennessee, Knoxville, Tenn.; Rebecca Quesnell, Zoetis, Kalamazoo, Mich.; Erin Royster, University of Minnesota, St. Paul, Minn.; Pamela Ruegg, University of Wisconsin, Madison, Wis.; K. Larry Smith, The Ohio State University, Wooster, Ohio; and Jennifer Timmerman, University of Minnesota, St. Paul, Minn.

Laboratory Handbook on Bovine Mastitis was NMC’s second publication. Thousands of copies have been distributed around the world.

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