Cattle Embryo Transfer Procedure: An Instructional Manual for the Rancher, Dairyman, Artificial Insemination Technician, Animal Scientist, and Veterinarian

Cattle Embryo Transfer Procedure: An Instructional Manual for the Rancher, Dairyman, Artificial Insemination Technician, Animal Scientist, and Veterinarian PDF

By John L. Curtis

Cattle Embryo Transfer Procedure: An Instructional Manual for the Rancher, Dairyman, Artificial Insemination Technician, Animal Scientist, and Veterinarian PDF. This comprehensive, step-by-step laboratory training manual brings all the elements for a successful embryo transfer program together in a simple, organized, illustrated format. For the last several decades, artificial insemination has allowed genetic progress to be achieved relatively quickly through the widespread and efficient use of frozen semen. As a result of the advancement of embryo transfer (ET) techniques, cows can produce many offspring. A more rapid genetic gain is achieved which complements an artificial insemination program.

Table of Contents

Preface
Acknowledgments
Overview of Embryo Transfer Procedure

1- Cattle Management
1.1 Health Program
1.2 Nutrition Program
2 Recipient Synchronization

3 Donor Superovulation and Artificial Insemination
3.1 Superovulation
3.2 Artificial Insemination

4- Embryo Recovery
4.1 Collection Date and Recipient Identification
4.2 Equipment and Donor Preparation
4.3 Embryo Collection

5- Embryo handling
5.1.Morphological Evaluation
5.2 Isolation and Extraction

6- Embryo Freezing
6.1. Equipment and Solutions
6.2 Embryo Selection, Washing and Freezing
6.3 Ccrtificatign, Identification and Labelling

7- Embryo Transfer
7.1 Thawing Frozen Embryos
7.2 Embryo Loading
7.3 Embryo Transfer

Appendix A: Embryo Collection and Transfer Supplies and Products
Appendix B: Embryo Freezing and Artificial Insemination Supplies and Products
Appendix C: Pharmaceuticals and Miscellaneous
Appendix D: Certificates and Forrns
Appendix E: Embryos and Ovaries
References

File Size6 MB
File FormatPDF
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