The Pentose Phosphate Pathway Terry Wood


The Pentose Phosphate Pathway Terry Wood

The Pentose Phosphate Pathway pdf

By Terry Wood

The Pentose Phosphate Pathway PDF Book aims to explore the pentose phosphate cycle and the practical techniques applied in its investigation. The main focus of the book is the pentose phosphate cycle in animals as well as microorganisms, and does not discuss the one related to photosynthesis. The book covers the formulation of the pathway, its types, and its alternative formulations; the preparation, processes, and analysis of the pathway; and the enzymes involved. Also covered in the book are the intermediates in intact cells and tissues; distribution of enzymes among different tissues and species; the operation, regulation, and overall control of the pathway; and the clinical, nutritional, and metabolic aspects of the pathway. The text is recommended for biologists and biochemists who would like to understand further the pentose phosphate pathway and the processes related to it.

Table of Contents


List of Abbreviations

1 Introduction


2 Formulation of the Pathway


Experiments with Isotopes

The L-Type Pathway

Other Alternative Formulations of the Pathway

D-Erythrose 4-Phosphate as an Intermediate of the Pathway

The Transketolase-Transaldolase Coupled Reaction and the Existence of Enzyme Complexes


3 Preparation, Properties, and Analysis of the Intermediates of the Pathway


General Analytical Methods

Preparation and General Chemistry

Individual Intermediates

4 Preparation, Properties, and Analysis of the Enzymes of the Pathway


D-Ribose-5-Phosphate Ketol-Isomerase (EC

D-Ribulose-5-Phosphate 3-Epimerase (EC

Transaldolase (EC

Transketolase (EC

Other Enzymes

5 Intermediates in Intact Cells and Tissues





Other Tissues

Ascites and HeLa Cells



Individual Intermediates

6 Distribution of the Enzymes among Different Tissues and Different Species


General Surveys

Specific Tissues

Measurements on Other Species

Changes in Enzyme Levels during Development


7 Operation and Regulation in Broken-Cell Preparations


The Utilization of Different Substrates

8 Operation and Regulation in Intact Cells and Tissues


Factors Stimulating the Oxidative Branch of the Pathway

The Effects of Changes in Physiological Factors on the Pathway

9 Overall Control of the Pathway


Approach I: Rate-Limiting Enzymes

Approach II: Mass Action Ratios

Approach III: Cross-Over Plots

Approach IV: Enzymes under Hormonal and Allosteric Control

Approach V: Enzymes That Change in Response to Changes in the Environment

Control of the Oxidative Branch of the Pathway by an Activating Factor

Mathematical Models


10 Clinical and Nutritional Aspects

The Assay of Blood Transketolase

Normal Values for Blood Transketolase

The “TPP Effect”

Measurements of Other Enzymes

Clinical Studies of Various Pathological and Physiological States

Studies of Transketolase and Thiamine Deficiency in Other Species

The Mechanism of Activation of Transketolase by Thiamine

11 Glutathione Metabolism and the Pentose Phosphate Pathway


12 Use of Radioisotopes in Elucidating the Nature of and Quantitating the Pentose Pathway


The Nature of the Pathway





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