Laboratory Exercises and Techniques in Cellular Biology
By Anthony Contento
Laboratory Exercises and Techniques in Cellular Biology PDF. This manual consists of 30+ experiments that coincide and complement each of the 18 chapters in the Karp text (978-1-1182-0673-7.) There are three possible designs of the lab book, based on the instructor’s needs. These designs focus on either Techniques, Concepts, or Organelles. The procedures of the 30+ experiments remain standard and unchanged in all designs of the lab book. Special Overview pages, Discussion Questions and Datasheets bookend the procedures in order to create each of the possible textbook designs. This gives instructors flexibility to create a lab book that suits their lecture course curriculum, their experience, and available equipment and supplies.
Table of Contents
Introduction vii
Acknowledgment ix
1 Introduction and Microscopy 1
(Karp chapter 1 & 18)
2 Enzyme Kinetics 11
(Karp chapter 3 & 11)
3 Membrane Permeability 19
(Karp chapter 4)
4 Protein Transport to the Plasma Membrane 27
(Karp chapter 4 & 8)
5 Cell Fractionation 33
(Karp chapter 5 & 12)
6 Isolation and Activity of Mitochondria 41
(Karp chapter 3 & 5)
7 Staining of the Extracellular Space 49
(Karp chapter 7)
8 Protoplast Isolation and Vacuole Staining 57
(Karp chapter 8)
9 Isolation of Vacuoles 65
(Karp chapter 8)
10 Staining of Autophagosomes and Lysosomes 71
(Karp chapter 8)
11 Staining of the Cytoskeleton 77
(Karp chapter 9)
12 Cell Motility 85
(Karp chapter 9)
13 Staining of the Nucleus and Nucleic Acids 95
(Karp chapter 12)
14 Staining of Chromosome and Cell Cycle Analysis 105
(Karp chapter 14)
15 Staining for Cell Viability and Apoptosis 113
(Karp chapter 15 & 16)
16 Staining of Immune Cells 121
(Karp chapter 17)
17 Protein Isolation, Fractionation, and SDS-PAGE 131
(Karp chapter 18)
18 Genomic DNA Isolation, Purification, and the Polymerase Chain Reaction 139
(Karp chapter 18)
19 Ligation and Cloning 145
(Karp chapter 18)
20 Restriction Fragment Length Polymorphism Analysis and Agarose Gel Electrophoresis 151
(Karp chapter 10 & 18)
