Introductory Experiments on Biomolecules and Their Interactions

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Introductory Experiments on Biomolecules and Their Interactions

Introductory Experiments on Biomolecules and Their Interactions PDF

By Robert K. Delong and Qiongqiong Zhou

Introductory Experiments on Biomolecules and their Interactions PDF provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further.

The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class.

Features quantitative analysis of RNA degradation by RNase
Contains problem sets, calculations, and references for each lab fully immersing students in the learning process
Includes instruction on how to maintain a lab notebook and write a formal lab report
Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied” examples of molecular interactions

Table of Contents

Introduction
About the Authors
Master Materials List
Format of the Lab Notebook and Formal Lab Report
Lab Safety and Policies
Experiment 1. An Introduction to Basic Math and Operations in the Biomolecular Laboratory
- Safety and Hazards
- Introduction
- Technical Review
- Equipment Highlight
- Experiment 1
- Methods and Procedure
- Results and Discussion
- Problem Set
Experiment 2. Preparing Buffers at a Specific Molarity and pH
- Safety and Hazards
- Introduction
- Technical Review
- Equipment Highlight
- Experiment 2
- Results and Discussion
- Problem Set
Experiment 3. Investigating the Physico-Chemical Properties of Amino Acids and Their Analysis by Thin Layer Chromatography
- Safety and Hazards
- Introduction
- Technical Review
- Equipment Highlight
- Experiment 3
- Methods and Procedure
- Results and Observations
- Discussion
- Problem Set
Experiment 4. Rapid Purification, Gel Electrophoresis, and Enzyme Activity Assay of the Luciferase Enzyme from Fireflies
- Safety and Hazards
- Introduction
- Technical Review and Equipment Highlights
- Experiment 4
- Results and Discussion
- Problem Set
Experiment 5. Hexokinase and G6PDH Catalyzed Reactions of Glucose Measurement
- Safety and Hazards
- Introduction
- Technical Review
- Equipment Highlight
- Experiment 5
- Results and Discussion
- Problem Set
Experiment 6. Polymerase Chain Reaction (PCR)
- Safety and Hazards
- Introduction
- Technical Review
- Experiment 6
- Results and Discussion
- Problem Set
Experiment 7. Investigating Protein:Nucleic Acid Interactions by Electrophoretic Mobility Shift Assay (EMSA)
- Safety and Hazards
- Introduction
- Equipment Highlights
- Experiment 7
- Results and Discussion
- Problem Set
Experiment 8. Qualitative Analysis of the Degradation of RNA via Ribonuclease A versus B
- Safety and Hazards
- Introduction
- Experiment 8
- Results and Discussion
- Problem Set
Experiment 9. Preparation of a Fluorescently Labeled Liposome and Its Analysis by Fluorescence Microscopy
- Safety and Hazards
- Introduction
- Experiment 9
- Results and Discussion
- Problem Set
Experiment 10. Studying Cell-like Structures with Liposome, DNA, and Protein
- Safety and Hazards
- Introduction
- Experiment 10
- Methods and Procedures
- Results and Discussion
- Additional Experiment
Index