Gnotobiotic Mouse Technology: An Illustrated Guide
By Chriss J. Vowles, Natalie E. Anderson, Kathryn A. Eaton
Gnotobiotic Mouse Technology: An Illustrated Guide PDF. The popularity of germ-free animal models, particularly mice, for investigation of human physiology and disease has recently exploded. Gnotobiotic Mouse Technology: An Illustrated Guide provides the first manual for the maintenance, husbandry, and experimental manipulation of germ-free and gnotobiotic mice. It includes information on all aspects of establishing and operating a germ-free mouse research facility, from basic principles and equipment to detailed instructions for assembling and maintaining isolators, sterilizing supplies, handling animals, and monitoring sterility.
The book breaks down techniques and procedures into modules, each of which contains a step-by-step practical and visual guide to a set of related procedures. Each description consists of a materials list, introduction, and general overview, followed by a thoroughly illustrated walkthrough of the steps for each procedure. The descriptions end with troubleshooting tips. Technical chapters are heavily illustrated and include notations of potential pitfalls and alternatives. Other chapters discuss management procedures and practices such as the hiring and training of personnel, setting fee schedules, and record keeping.
This book is a landmark resource for establishing and maintaining a facility for germ-free mouse research. Useful to both technicians and investigators, it presents every step necessary to establish a successful facility. It also gives direction in expanding multi-user facilities and applying new technologies to your current practices.
Overview of Gnotobiotic Technology Overview
How Do We Get Germ-Free Mice?
Germ-Free Housing and Barrier Types
References
Equipment and Terminology
Personal Protective Equipment
Introduction
Types of PPE
Applying Sterile PPE
Sterilants and Sterilization Introduction
Sterilization Methods
Cold Sterilization
Wrapping Materials for Autoclaving
Gas Sterilization (Ethylene Oxide)
Isolator Setup Introduction
Part 1: Small (5.5 x 3 feet) Isolator Assembly
Part 2: Large Isolator Assembly
Port Entry and Exit Introduction
Supplies
Preparing, Opening, and Closing the Port
Proper Placement of Materials
Sterilizing Food and Supplies Introduction
Biological Indicators
Assembling Supply Cylinders: Installing HEPA Filtration Media
Supplies
Prepare the Cylinder
Fill the Cylinder
Water Sterilization
Isolator Maintenance Introduction
Part 1: Cleaning and Maintenance
Part 2: Replacing Gloves on an Active Isolator
Part 3: Repairing Holes in Isolators
Part 4: Working in the Isolator
Aseptic Mouse Transfer Introduction
Part 1: Internal Transfer between Isolators
Part 2: External Transfer of Mice
Working with Germ-Free or Gnotobiotic Mice in the Class II Biosafety Cabinet Introduction
Supplies
Experimental Manipulations in the Biosafety Cabinet
Shipping Mice Introduction
Procedure
Rederivation Introduction
Derivation by Cesarean Section
Derivation by Embryo Transfer
References
Microbiological Testing Introduction
Serologic Screening
Part 1: Bacterial Culture
Part 2: Examination of Gram-Stained Slides for the Presence of Bacteria
References
Genetic Testing References
Record Keeping Introduction
Colony Management Documents
Project-Planning Documents
Other Documents
Facility Setup and Management Introduction
Establishing a New Facility
