Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

Features:

• Includes cutting-edge methods and protocols
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts

Table of Contents:

PART I – Introducing Nucleic Acid Testing in the Veterinary Laboratory

1. Overview and Challenges of Molecular Technologies in the Veterinary Microbiology Laboratory

2. Significance and Integration of Molecular Diagnostics in the Framework of Veterinary Practice

3. Biosafety Principles and Practices for the Veterinary Diagnostic Laboratory

4. Veterinary Biobank Facility: Development and Management for Diagnostic and Research Purposes

5. Biological Specimen Collection and Processing for Molecular Analysis

6. Validation of Molecular Diagnostic Assays and Quality Assurance and Control in the Veterinary Laboratory

PART II – Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings

7. Molecular Approaches to Recognize Relevant and Emerging Infectious Diseases in Animals

8. Real-Time Reverse Transcriptase PCR for the Detection of Bluetongue Virus

9. Nested and Multiplex Real-Time PCR Using Dual-Labeled Probes: Detecting and Discriminating Mycobacterium tuberculosis Complex Members in Cultures and Animal Tissues

10. A Real-Time PCR Assay for the Diagnosis of Gastrointestinal Nematode Infections of Small Ruminants

11. Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches

12. Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Technique

13. Direct Detection of Theileria annulata in Bovine Blood Samples Using Standard and Isothermal DNA Amplification Approaches

14. Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection

15. DNA Microarray-Based Detection of Multiple Pathogens: Mycoplasma spp. and Chlamydia spp.

16. In Situ Hybridization with Labeled Probes: Assessment of African Swine Fever Virus in Formalin-Fixed Paraffin-Embedded Tissues

17. Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections

18. Identification of Animal Pasteurellaceae by MALDI-TOF Mass Spectrometry

19. Gold Nanoparticles as a Potential Tool for Diagnosis of Fish Diseases

20. Nucleic-Acid Testing, New Platforms and Nanotechnology for Point-of-Decision Diagnosis of Animal Pathogens

PART III – Molecular Profiling of Veterinary Relevant Microbial Pathogens

21. Molecular Typing Tools: From Pattern Recognition to Genome-Based Algorithms

22. Characterization of Campylobacter jejuni and Campylobacter coli Genotypes in Poultry Flocks by Restriction Fragment Length Polymorphism (RFLP) Analysis

23. Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci

24. Multiple-Locus Variable-Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR and Multicolor Capillary Electrophoresis: Application to the Genotyping of Brucella Species

25. Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains

26. Single-Nucleotide Polymorphism Discrimination Using High-Resolution Melting Analysis for the Genotyping of Bacillus anthracis

27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) Analysis of Members of the Mycobacterium tuberculosis Complex

28. Rapid Microarray-Based Genotyping of Chlamydia spp. Strains from Clinical Tissue Samples

29. Multiplexed Genotyping of Bacillus anthracis by Luminex xMap Suspension Array

PART IV – Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction with the Host

30. Next-Generation Sequencing in Veterinary Medicine: How Can the Massive Amount of Information Arising from High-Throughput Technologies Improve Diagnosis, Control and Management of Infectious Diseases?

31. Impact of Next-Generation Technologies on Exploring Socioeconomically Important Parasites and Developing New Interventions

32. Functional Genomics of Tick Vectors Challenged with the Cattle Parasite Babesia bigemina

33. Metagenomic Approaches to Disclose Disease-Associated Pathogens: Detection of Viral Pathogens in Honeybees

34. Proteomics Characterization of Tick-Host-Pathogen Interactions

Index