Principles of Animal Cell Culture Students Compendium

Principles of Animal Cell Culture Students Compendium PDF

By Kumar Rinesh Sinha Basant K

Principles of Animal Cell Culture Students Compendium PDF. Animal cell culture is playing a pivotal role in the various modern researches. Though the foundation stone of animal cell culture was laid for the isolation and characterization of different viruses but the recent developments in the field of biotechnology and molecular biology have given various new dimensions to this technology. Cell culture has provided a new tool for the better understanding of diagnosis and molecular biology in many areas of biological systems including bacterial, viral and parasitic diseases. The different cell cultures are being used in virology for the last five decades and one of its application has been utilized successfully in various types of vaccine production. To quote virologist Payton Rous of late we have begun to study viruses not only for what they themselves mean, but for what they can reveal about the intrinsic cell structure, and functions through located injuries that they do”. More recent discoveries such as production of various proteins and enzymes through recombinant DNA technology require the use of cell culture to study the exact gene expression, have fully utilized where the available evidences justify it and where their use is consistent with the effort involved and their routine application is recommended. With the advancement in tissue culture technology, scientists are now on the threshold of bringing sea changes in improving the life of human as well as livestock and thus it has become a source of unending fascination for different scientists.

The present work is directed primarily to the students of biotechnology, keeping in view the University Grants Commission Syllabus on animal cell culture prescribed for vocational course both for undergraduate and postgraduate students and also for the scientists in the medical, veterinary and biological sciences, whose curiosity and efforts are responding to the stimulus of current interest in biotechnology.

Table of Contents

Chapter 1 History of Development of Cell Culture

Chapter 2 Natural Surroundings of Animal Cell

Chapter 3 Metabolic Capabilities of Animal Cell

Chapter 4 Simulating Natural Conditions for Growing Animal Cells

Chapter 5 Serum and Serum Free Media

Chapter 6 Cell Culture System

Chapter 7 Secondary Culture: Transformed Animal cell

Chapter 8 Commonly used Cell Lines and their Uses

Chapter 9 Growth Kinetics of Cells in Culture

Chapter 10 Gene Expression and Application of Animal Cell Culture for Its Studies

Chapter 11 Organ Culture

Chapter 12 Transfection

Chapter 13 Cell Fusion

Chapter 14 Selectable Marker andAntibiotic Resistance

Chapter 15 Hybridoma and Monoclonal Antibodies

Chapter 16 Transplantation of Cultured Cell

Chapter 17 Differentiation of Cells

Chapter 18 Transgenesis and Transgenic Animals

Chapter 19 Apoptosis, Necrosis, Senescence and Quiescence

Chapter 20 Embryo Transfer Technology

References

Index

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