Cellular and Molecular Approaches in Fish Biology

Features:

Table of Contents:

Chapter 1: Technologies and strategies for ex situ conservation of aquatic organisms: The role of cryopreservation in long-term management

• Abstract
• Acknowledgments
•  Factors and steps to consider in setting up a cryopreservation protocol
•  Applications of cryopreservation techniques in aquatic animals
•  Cryopreservation of different biological materials to create Genome Resource Banks
•  Examples of sperm cryopreservation in specific species
• Conclusions and future perspectives
• References
• Further reading

Chapter 2: Molecular approaches on DNA damage evaluation after primordial germ cell cryopreservation in zebrafish

• Abstract
• Acknowledgments
•  Introduction
•  Conventional DNA evaluation methods
•  DNA lesion detection by qPCR: A novel approach
•  Our contribution to DNA damage evaluation in cryopreserved germ cells
•  Specific protocol for DNA damage evaluation in zebrafish PGCs after cryopreservation
•  Summary and future perspectives
• References

Chapter 3: The role of epigenetics in fish biology and reproduction: An insight into the methods applied to aquaculture

• Abstract
•  Introduction: Concept and importance of epigenetics in terms of evolution
•  Epigenetic mechanisms
•  The role of epigenetics in farmed fish species
•  Epigenetic mechanisms in model species: A case study in zebrafish
•  Techniques applied for the evaluation of DNA methylation
•  Techniques applied for the evaluation of histone modifications
• Application of the epigenetic methods to our research
• References
• Further reading

Chapter 4: Noncoding RNAs in fish physiology and development: miRNAs as a cornerstone in gene networks

• Abstract
• Acknowledgments
•  Noncoding RNAs
•  Biogenesis and mechanisms of action of miRNAs
•  Methodological approaches applied in miRNA research
•  miRNAs playing key roles in fish development and physiology
•  Recent progress and future perspectives
• References

Chapter 5: Nutritional epigenetics

• Abstract
•  Introduction
•  Epigenetic regulators
•  Nutrients and metabolites influencing the epigenetic regulators
• Transfer of cell memory utilizing the epigenetic regulators
• Where do we go with nutritional epigenetics?
• References
• Chapter 6: Methods for mapping genomic variants associated with production traits in aquaculture species
• Abstract
• Introduction
•  Finding causative variants through QTL mapping approaches
•  Detailed description of state-of-the-art methodology: Step by step on how to perform GWAS
• Pipeline
•  The next generation of association mapping methods: Multimarker models
•  Implementation of QTLs and causative variants into breeding programs
•  Funneling candidate variants toward causality
•  Conclusions
• References

Chapter 7: Double-labeling immunofluorescence: A simple and effective tool to study pituitary regulation in teleost fish

• Abstract
• Acknowledgments
•  The importance of gaining knowledge about the neuroendocrine system
•  Neuroendocrine syste Double-labeling immunofluorescence
•  Going deeper on the pituitary hormone regulation
•  How to approach this kind of neuroendocrine study in fish?
•  Summary
• References

Chapter 8: Mucosal immunology in fish

• Abstract
• Acknowledgments
•  A brief introduction to the immune system in teleost fish
•  Historical perspective and relevance of immunological studies in gilthead seabream
•  Fish mucosal immunology
•  How to alter or strengthen the fish mucosal immune system
•  Methodologies to collect and preserve fish mucus
•  Main questions still to be addressed
• References

Chapter 9: Characterization of the neuroendocrine stress status as part of the multiparametric assessment of welfare in fish

• Abstract
•  Animal welfare
•  The neuroendocrine stress response and its relation to welfare
•  A proposed method to determine the stress status of fishes as part of the assessment of their welfare
•  Conclusions and future perspectives
• References

Chapter 10: Evolution of ray-finned fish genomes: Status and directions with a primer on microRNA characterization

• Abstract
•  Introduction
•  Resources to study fish genome evolution
•  Overview of fish genome evolution
• Evolutionary consequences of rediploidization—No “one size fits all”
• Transposable elements as agents of fish genome evolution
•  Fish phylogenomics
•  Evolution of “functional” regions in fish genomes
•  A primer on miRNA evolutionary characterization
•  Concluding remarks
• References
• Chapter 11: Zebrafish xenotransplantation: A versatile platform for cancer and stem cell translational research
• Abstract
•  Introduction
•  Larval zebrafish XT models of cancer
•  Using the zebrafish for translational stem cell research
•  Adult zebrafish XT models of cancer
•  Conclusion
• References
• Chapter 12: Status, challenges, and perspectives of fish cell culture—Focus on cell lines capable of in vitro mineralization
• Abstract
• Acknowledgments
•  A growing interest for cell lines of fish origin
•  Current status of fish cell culture
•  Applications for fish cell lines
•  Fish cell lines capable of in vitro mineralization
•  Development of bone-derived cells
• Perspectives for fish cell culture
• References
• Index