Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies

Features:

• Includes cutting-edge methods and protocols
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts

• PART I – Introducing Nucleic Acid Testing in the Veterinary Laboratory
• Overview and Challenges of Molecular Technologies in the Veterinary Microbiology Laboratory
• Significance and Integration of Molecular Diagnostics in the Framework of Veterinary Practice
• Biosafety Principles and Practices for the Veterinary Diagnostic Laboratory
• Veterinary Biobank Facility: Development and Management for Diagnostic and Research Purposes
• Biological Specimen Collection and Processing for Molecular Analysis
• Validation of Molecular Diagnostic Assays and Quality Assurance and Control in the Veterinary Laboratory
• PART II – Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings
• Molecular Approaches to Recognize Relevant and Emerging Infectious Diseases in Animals
• Real-Time Reverse Transcriptase PCR for the Detection of Bluetongue Virus
• Nested and Multiplex Real-Time PCR Using Dual-Labeled Probes: Detecting and Discriminating Mycobacterium tuberculosis Complex Members in Cultures and Animal Tissues
• A Real-Time PCR Assay for the Diagnosis of Gastrointestinal Nematode Infections of Small Ruminants
• Improved Detection of Mycobacterium bovis in Bovine Tissues Using Immunomagnetic Separation Approaches
• Detection of Fish Pathogens by Loop-Mediated Isothermal Amplification (LAMP) Technique
• Direct Detection of Theileria annulata in Bovine Blood Samples Using Standard and Isothermal DNA Amplification Approaches
• Reverse Line Blot Hybridization with Species-Specific Oligonucleotide Probes: Application to Piroplasm Detection
• DNA Microarray-Based Detection of Multiple Pathogens: Mycoplasma spp. and Chlamydia spp.
• In Situ Hybridization with Labeled Probes: Assessment of African Swine Fever Virus in Formalin-Fixed Paraffin-Embedded Tissues
• Fluorescence In Situ Hybridization for the Tissue Detection of Bacterial Pathogens Associated with Porcine Infections
• Identification of Animal Pasteurellaceae by MALDI-TOF Mass Spectrometry
• Gold Nanoparticles as a Potential Tool for Diagnosis of Fish Diseases
• Nucleic-Acid Testing, New Platforms and Nanotechnology for Point-of-Decision Diagnosis of Animal Pathogens
• PART III – Molecular Profiling of Veterinary Relevant Microbial Pathogens
• Molecular Typing Tools: From Pattern Recognition to Genome-Based Algorithms
• Characterization of Campylobacter jejuni and Campylobacter coli Genotypes in Poultry Flocks by Restriction Fragment Length Polymorphism (RFLP) Analysis
• Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci
• Multiple-Locus Variable-Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR and Multicolor Capillary Electrophoresis: Application to the Genotyping of Brucella Species
• Multilocus Sequence Typing (MLST): Markers for the Traceability of Pathogenic Leptospira Strains
• Single-Nucleotide Polymorphism Discrimination Using High-Resolution Melting Analysis for the Genotyping of Bacillus anthracis
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) Analysis of Members of the Mycobacterium tuberculosis Complex
• Rapid Microarray-Based Genotyping of Chlamydia spp. Strains from Clinical Tissue Samples
• Multiplexed Genotyping of Bacillus anthracis by Luminex xMap Suspension Array
• PART IV – Integrative Omics and High-Throughput Platforms
• Next-Generation Sequencing in Veterinary Medicine
• Impact of Next-Generation Technologies on Exploring Socioeconomically Important Parasites and Developing New Interventions
• Functional Genomics of Tick Vectors Challenged with the Cattle Parasite Babesia bigemina
• Metagenomic Approaches to Disclose Disease-Associated Pathogens: Detection of Viral Pathogens in Honeybees
• Proteomics Characterization of Tick-Host-Pathogen Interactions
• Index