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Experiments in the Purification and Characterization of Enzymes A Laboratory Manual

Experiments in the Purification and Characterization of Enzymes A Laboratory Manual

Experiments In The Purification And Characterization Of Enzymes A Laboratory Manual

Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material.

Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics.

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Ferritin Functions, Biosynthesis and Regulation

Ferritin Functions, Biosynthesis and Regulation

Ferritin Functions Biosynthesis And Regulation

Ferritins are a superfamily of iron-storage proteins that are found in all living organisms with the exception of yeast. They are ubiquitous, iron-binding proteins that play an important role in cellular iron homeostasis. This book discusses topical research in the study of the functions, biosynthesis and regulation of ferritin. Topics include ferritin as an iron source for pathogens and as part of the antioxidative machinery in plants under stress; the antioxidant role of ferritin and transferrin in elite athletes; ferritin and ferroxidase activity and increased serum ferritin in morbid obesity and bariatric surgery.

 

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Functional Metabolism Regulation and Adaptation

Functional Metabolism Regulation and Adaptation

Functional Metabolism Regulation And Adaptation

Functional Metabolism of Cells is the first comprehensive survey of metabolism, offering an in-depth examination of metabolism and regulation of carbohydrates, lipids, and amino acids. It provides a basic background on metabolic regulation and adaptation as well as the chemical logic of metabolism, and covers the interrelationship of metabolism to life processes of the whole organism. The book lays out a structured approach to the metabolic basis of disease, including discussion of the normal pathways of metabolism, altered pathways leading to disease, and use of molecular genetics in diagnosis and treatment of disease. It also takes a unique comparative approach in which human metabolism is a reference for metabolism in microorganisms and plant design, and presents novel coverage of development and aging, and human health and animal adaptation. The final chapter reviews the past and future promise of new genetic approaches to treatment and bioinformatics.
This, the most exhaustive treatment of metabolism currently available, is a useful text for advanced undergraduates and graduates in biochemistry, cell/molecular biology, and biomedicine, as well as biochemistry instructors and investigators in related fields.

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General Enzymology

General Enzymology

General Enzymology

By Kulkarni and deshpande

General Enzymology is a comprehensive text that can be used by students and teachers of Biochemistry, Microbiology, Biotechnology and Medical Laboratory Technology. The text has easy, understandable language and covers almost all topics on enzymes, that are a part of cirricula of most Indian Universities. A large number of diagrammatic illustrations make the understanding of text easy. Points have been summarized with the help of table wherever necessary. About 50 terms and definitions commonly used in relation to enzymes have been included. The chapter on enzyme kinetics is simple enough.

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General, Organic, and Biochemistry An Applied Approach

General, Organic, and Biochemistry: An Applied Approach PDF

General, Organic, and Biochemistry An Applied Approach [PDF Download]

General, Organic, And Biochemistry: An Applied Approach Pdf

By James Armstrong

Focusing on key topics important in allied health and nursing careers, General, Organic, and Biochemistry An Applied Approach PDF is ideal for readers who have had no prior exposure to chemistry. Emphasizing problem-solving techniques, the book takes the most direct path to biomolecules and metabolic processes, provides a wealth of worked examples to help readers understand key chemical concepts, includes novel and relevant “Health Notes” in the margins, and weaves biological and medical applications throughout.

Table of Contents
  • 1. Measurements in Science and Medicine
    • Measuring Size: Distance, Mass, and Volume
    • Measurements in Science: Precision and Accuracy
    • Metric Units and Their Relationships
    • Unit Conversions and Conversion Factors
    • Using Multiple Conversion Factors
    • Density, Dosage, and Other Compound Units
    • Temperature
    • Connections: Why Do We Struggle Against the Metric System?
  • 2. Atoms, Elements, and Compounds
    • Classifying Matter: Mixtures, Compounds, and Elements
    • The Chemical Elements: An Introduction to the Periodic Table
    • Atoms
    • Subatomic Particles and Atomic Structure
    • Isotopes and Atomic Weight
    • Chemical Formulas
    • Electron Shells
    • Chemical Behavior, Valence Electrons, and the Periodic Law
    • The Organization of the Periodic Table
    • Connections: The Elements of Life
  • 3. Chemical Bonds
    • Covalent Bonds and the Octet Rule
    • Double and Triple Bonds
    • Electronegativity and Polar Bonds
    • Naming Covalent Compounds
    • Ions and Ionic Compounds
    • Writing Formulas for Ionic Compounds
    • Naming Ionic Compounds
    • Polyatomic Ions
    • Recognizing Ionic and Molecular Compounds
    • Connections: Nitrogen and Oxygen: A Remarkable Partnership
  • 4. Energy and Physical Properties
    • Heat and Energy
    • The Three States of Matter
    • The Properties of Gases
    • Changes of State
    • Attractive Forces and the Physical Properties of Matter
    • Solutions and the Dissolving Process
    • Electrolytes and Dissociation
    • Solubility
    • The Relationship between Solubity and Molecular Structure
    • Connections: Temperature, Pressure, and Volume in Everyday Life
  • 5. Solution Concentration
    • Concentration
    • Moles and Formula Weights
    • Molarity
    • Osmosis, Dialysis, and Tonicity
    • Equivalents
    • Dilution
    • Connections: Physiological Dehydration

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Glutamic Acid Chemistry, Food Sources and Health Benefits

Glutamic Acid Chemistry, Food Sources and Health Benefits

Glutamic Acid Chemistry Food Sources And Health Benefits

Poly (y-glutamic acid), a novel polyanionic and multifunctional macromolecule synthesised by Bacillus species, has attracted considerable attention because of its eco-friendly, biodegradable and biocompatible characteristics. Recently, its application in a wide range of fields such as food, agriculture, medicine, hygiene, cosmetics and the environment has been explored. This book discusses the chemistry, food sources and health benefits of glutamic acid. Topics include the self-organization and stability of poly a-glutamic acid with cationic surfactants; metabolism and physiology of glutamate in chickens; different roles and applications of glutamic acid in the biomedical field; glutamic acid in food and its thermal degradation in acidic medium; application of a natural biopolymer poly (y-glutamic acid) as a bioflocculant and adsorbent for cationic dyes and chemical mutagens; and biodegradability of the y- and a-enantiomeric forms of the poly (glutamic acid) by bacillus licheniformis NCIMB 11709.

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Glutathione Biochemistry, Mechanisms of Action and Biotechnological Implications

Glutathione Biochemistry, Mechanisms of Action and Biotechnological Implications

Glutathione Biochemistry Mechanisms Of Action And Biotechnological Implications

Glutathione (glutamyl-cysteinyl-glycine; GSH) is the most abundant low-molecular-weight thiol found in both prokaryotic and eukaryotic organisms. This text discusses cutting-edge developments and deals with the information gained over the last few years on the physiological, biochemical and molecular roles of the GSH and its significance in mammalian, microbial and plant cells.

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Glycine Biosynthesis, Physiological Functions and Commercial Uses

Glycine Biosynthesis, Physiological Functions and Commercial Uses

Glycine Biosynthesis Physiological Functions And Commercial Uses

In this book, the authors present current research in the study of the biosynthesis, physiological functions and commercial uses of glycine. Topics discussed include the molecular complexation of glycine with 18-crown-6 in aqueous-organic solvent; concentrated glycine based electrolytes and application to solid pH sensor; heterologous expression of the glycine receptor chloride channel; and the theoretical evaluation of the properties of energy deposition associated with the collision of fast ions with glycine and its zwitterions.

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Handbook of Proteolytic Enzymes, 3 Volumes, 3rd Edition

Handbook of Proteolytic Enzymes, 3 Volumes, 3rd Edition

Handbook Of Proteolytic Enzymes 3 Volumes 3Rd Edition

Extensively revised and updated, the new edition of the highly regarded Handbook of Proteolytic Enzymes is an essential reference for biochemists, biotechnologists and molecular biologists. Edited by world-renowned experts in the field, this comprehensive work provides detailed information on all known proteolytic enzymes to date. This two-volume set unveils new developments on proteolytic enzymes which are being investigatedin pharmaceutical research for such diseases as HIV, Hepatitis C, and the common cold.

Volume I covers aspartic and metallo petidases while Volume II examines peptidases of cysteine, serine, threonine and unknown catalytic type. A CD-ROM accompanies the book containing fully searchable text, specialised scissile bond searches, 3-D color structures and much more.

  • The only comprehensive book on proteolytic enzymes
  • Includes 671 chapters, each written by experts in their field, on proteolytic enzymes from all groups of living organisms and the viruses, including those that are currently major targets of pharmaceutical research
  • Accompanying CD-ROM provides fully searchable text, 2D structures of peptidases in color and links directly to PubMed and MEROPS databases
  • Each chapter describes in detail the enzyme name, its history, activity and specificity, structural chemistry, preparation, biological aspects and distinguishing features
  • Over 1000 peptidases included

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High-Yield Biochemistry

High-Yield Biochemistry

High Yield Biochemistry

Part of the popular High-Yield&; Series , this updated Third Edition provides a succinct, heavily illustrated review in outline format of medical biochemistry for students preparing for USMLE Step 1. Highlights of this edition include revised and updated chapters on gene expression and biomedical technology. More clinical correlates have been added throughout the text.

 

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