Confocal Microscopy for Biologists

Confocal Microscopy for Biologists PDF

By Alan R. Hibbs

Confocal Microscopy for Biologists PDF. There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.
Table of Contents

1. WHAT IS CONFOCAL MICROSCOPY

2. UNDERSTANDING MICROSCOPY

3. CONFOCAL MICROSCOPY HARDWARE

4. IMAGE COLLECTION

5. DIGITAL IMAGES IN MICROSCOPY

6. IMAGING SOFTWARE

7. PRESENTATION AND PUBLICATION

8. WHAT IS FLUORESCENCE

9. FLUORESCENT PROBES

10. CONFOCAL MICROSCOPY TECHNIQUES

11. FLUORESCENCE IMMUNOLABELLING

12. IMAGING LIVE CELLS

13. THE INTERNET

14. TECHNICAL SUPPLIES

15. FURTHER READING

APPENDIX 1: CONFOCAL MICROSCOPES

A. BIO-RAD CELL SCIENCE DIVISION

CARL ZEISS MICROSCOPY

LEICA MICROSYSTEMS

NIKON INSTRUMENTS

OL YMPUS CORPORATION

ATTO BIOSCIENCE

YOKOGAWA ELECTRIC CORPORATION

PERKINELMER LIFE SCIENCES

VISITECH INTERNATIONAL

GLOSSARY

INDEX

File Size63.3 MB
File FormatPDF
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